NIGHTSEA DFP Dual Fluorescent Protein Excitation Flashlight
The Model DFP Dual Fluorescent Protein Flashlights include two different, switch-selectable high intensity LEDs for excitation of fluorescent proteins. For years the most popular fluorescent colors have been green (GFP, eGFP, fluorescein, etc.) and red (DsRed, TdTomato, etc.), and we offered only one version of the DFP, with a combination of blue and green LEDs for exciting these fluorophores. This is still our ‘standard’ DFP product, but we are now offering build-to-order combinations using any two of the excitation colors that are available with our popular Model SFA Stereo Microscope Fluorescence Adapter.
With every DFP light you will receive two pairs of barrier filter glasses and a convenient storage case. The glasses are well matched to the excitation so that they block the reflected excitation light while transmitting the fluorescence with high efficiency, providing excellent viewing contrast.
This is an convenient alternative to built in fluorescence for your microscopy and portable live fluorescent viewing needs.
Specifications:
Dimensions: 5 x 3 x 2
Battery Type: 4 C-cell
Bulb Type: Two high intensity 3W LED
Burn Time: 4 hours at full power
Lamp Life: 10,000 hours
Matching filter glasses and carrying case Included
Filter Set | Excitation | Emission | Fluorophores | |
RB – Royal Blue | 440-460nm | 500nm LP | GFP, fluorescein, lucifer yellow, … | |
CY – Cyan | 490-515nm | 550nm LP | YFP, Venus, … | |
GR – Green | 510-540nm | 600nm LP | DsRed, TdTomato, RFP, … | |
VI – Violet | 400-415nm | 460nm LP | BFP, CFP, … | |
UV – Ultraviolet | 360-380nm | 415nm LP | DAPI, …
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http://www.luyorgroup.com/Fluorescent-Protein-light-sources/LUYOR-3280-Fluorescence-Flashlight.html
http://www.luyorgroup.com/Fluorescent-Protein-light-sources/LUYOR-3415RG.html
DFP-1熒光手電筒只需一個簡單的手電筒即可在您的轉基因實驗中篩選綠色和紅色熒光蛋白。The NightSea DFP-1 雙熒光蛋白手電筒包括兩個 3W 高強度發(fā)光二極管,每個都配有定制的干涉濾光片。將開關單向轉動,您有藍光來激發(fā) GFP,將它轉向另一個方向,強烈的綠光激發(fā) DsRed。DFP-1 帶有兩對阻隔濾光玻璃——一個與每個 LED 一起使用——可阻擋反射光并傳輸熒光。
規(guī)格
電池:4 C 電池
燈泡類型:兩個高強度3W LED
燃燒時間:>4小時全功率
LED燈壽命:>10,000小時
尺寸:5 英寸(317.5 像素)長 x 3 英寸(7.6 厘米)直徑(不包括手槍式握把)
DFP-1熒光手電筒。
DFP-1 雙熒光蛋白手電筒,美國Nightsea生產
DFP-1 Dual Fluorescent Protein Flashlight
DFP-1 雙熒光蛋白手電筒的應用:
用于檢測、篩選轉綠色熒光蛋白(GFP)和紅色熒光蛋白(DsRed)的植物、動物及微生物,如水稻、玉米、斑馬魚、小鼠、細菌、真菌等等。。。。
DFP-1 雙熒光蛋白手電筒包含:雙熒光手電筒、黃色濾光眼鏡、紅色濾光眼鏡三個組件
DFP-1 雙熒光蛋白手電筒組件的使用:
1.雙熒光手電筒(激發(fā)光源):手電筒狀激發(fā)光源可激發(fā)兩種光源:
1)3W LED Royal Blue——400-460nm,藍色光源,照射含綠色熒光蛋白的樣本;
2)3W LED Green——510nm-540nm,綠色光源,照射含紅色熒光蛋白的樣本。
2.濾光眼鏡(眼鏡兩個):
選擇藍色光源激發(fā)光或綠色光源激發(fā)光照射樣本后,
1)黃色濾光鏡觀測,濾光鏡擋住反射光,只允許綠色熒光通過,從而可以觀測到綠色熒光蛋白;
2)紅色濾光鏡觀測,濾光鏡擋住反射光,只允許紅色熒光通過,從而可以觀測到紅色熒光蛋白
注:
DFP-1 雙熒光蛋白手電筒的標配是檢測綠色熒光蛋白(GFP)和紅色熒光蛋白(DsRed)
現在,您可以選擇以下任何兩種熒光裝置,組裝成您專屬的DFP-xy 雙熒光蛋白手電筒啦。
熒光裝置型號 | citation激發(fā)光 | Emission發(fā)射光 | 可觀測熒光 |
RB-Royal Blue | 440-460nm | 500nm LP | GFP,eGFP,fluorescein.... |
CY-Cyan | 490-515nm | 550nm LP | YFP,Venus,Lucifer Yellow... |
GR-Green | 510-540nm | 600nm LP | DsRed,dTomato... |
VI-Violet | 400-415nm | 460nm LP | CFP,BFP... |
UV-Ultra Violet | 360-380nm | 415nm LP | DAPI |
DFP-1雙熒光蛋白手電筒的產品優(yōu)勢:
1.小巧、攜帶方便,便于野外檢測
2.檢測效率高,黑暗中檢測一目了然
3.LED壽命10000h,且系統(tǒng)穩(wěn)定,可用于長時間(>4h)使用
4.直接檢測,而無需用底物顯色,所以安全,對被檢測對象無傷